Kinase domain autophosphorylation rewires the activity and substrate specificity of CK1 enzymes

Mol Cell. 2022 Jun 2;82(11):2006-2020.e8. doi: 10.1016/j.molcel.2022.03.005. Epub 2022 Mar 29.


CK1s are acidophilic serine/threonine kinases with multiple critical cellular functions; their misregulation contributes to cancer, neurodegenerative diseases, and sleep phase disorders. Here, we describe an evolutionarily conserved mechanism of CK1 activity: autophosphorylation of a threonine (T220 in human CK1δ) located at the N terminus of helix αG, proximal to the substrate binding cleft. Crystal structures and molecular dynamics simulations uncovered inherent plasticity in αG that increased upon T220 autophosphorylation. The phosphorylation-induced structural changes significantly altered the conformation of the substrate binding cleft, affecting substrate specificity. In T220 phosphorylated yeast and human CK1s, activity toward many substrates was decreased, but we also identified a high-affinity substrate that was phosphorylated more rapidly, and quantitative phosphoproteomics revealed that disrupting T220 autophosphorylation rewired CK1 signaling in Schizosaccharomyces pombe. T220 is present exclusively in the CK1 family, thus its autophosphorylation may have evolved as a unique regulatory mechanism for this important family.

Keywords: CK1; Schizosaccharomyces pombe; casein kinase 1; phosphoregulation; protein kinase regulation; quantitative phosphoproteomics; structural biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Casein Kinase Idelta
  • Humans
  • Phosphorylation
  • Protein Serine-Threonine Kinases*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Signal Transduction
  • Substrate Specificity
  • Threonine


  • Threonine
  • Casein Kinase Idelta
  • Protein Serine-Threonine Kinases