Five Hours Total Sleep Deprivation Does Not Affect CA1 Dendritic Length or Spine Density

Alvin T S Brodin; Sarolta Gabulya; Katrin Wellfelt; Tobias E Karlsson

doi:10.3389/fnsyn.2022.854160

Five Hours Total Sleep Deprivation Does Not Affect CA1 Dendritic Length or Spine Density

Front Synaptic Neurosci. 2022 Mar 14:14:854160. doi: 10.3389/fnsyn.2022.854160. eCollection 2022.

Authors

Alvin T S Brodin¹, Sarolta Gabulya², Katrin Wellfelt¹, Tobias E Karlsson¹

Affiliations

¹ Department of Neuroscience, Karolinska Institutet, Solna, Sweden.
² Institute of Neuroinformatics, University of Zurich and ETH, Zürich, Switzerland.

Abstract

Sleep is essential for long term memory function. However, the neuroanatomical consequences of sleep loss are disputed. Sleep deprivation has been reported to cause both decreases and increases of dendritic spine density. Here we use Thy1-GFP expressing transgenic mice to investigate the effects of acute sleep deprivation on the dendritic architecture of hippocampal CA1 pyramidal neurons. We found that 5 h of sleep deprivation had no effect on either dendritic length or dendritic spine density. Our work suggests that no major neuroanatomical changes result from a single episode of sleep deprivation.

Keywords: dendrite; hippocampus; plasticity; sleep deprivation; spines.