KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28

EMBO J. 1986 Oct;5(10):2697-701.

Abstract

We have isolated, in yeast, a nuclear gene named KIN28 which presents significant sequence homology with the cell-division-cycle CDC28 gene, with members of the protein-tyrosine kinase family (src, erb, abl, epidermal growth factor, etc.) and those of the family of protein kinases phosphorylating serine and threonine. This strongly suggests that KIN28 is endowed with a protein kinase activity. In contrast with CDC28, KIN28 is interrupted by an intervening sequence. The KIN28 gene failed to complement cdc28 mutations and was shown to be essential for cell proliferation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Nucleus / physiology
  • Cyclin-Dependent Kinases / genetics*
  • Genes*
  • Genes, Fungal*
  • Protein Kinases / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • Cyclin-Dependent Kinases
  • Kin28 protein kinase, S cerevisiae

Associated data

  • GENBANK/X04423