Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jun;121(6):1749-1760.
doi: 10.1007/s00436-022-07508-5. Epub 2022 Apr 2.

Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase from Eimeria tenella

Affiliations

Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase from Eimeria tenella

Wenhao Huang et al. Parasitol Res. 2022 Jun.

Abstract

Chicken coccidiosis is an extremely common and lethally epidemic disease caused by Eimeria spp. The control measures of coccidiosis depend mainly on drugs. However, the ensuing drug resistance problem has brought considerable economic loss to the poultry industry. In our previous study, comparative transcriptome analyses of a drug-sensitive (DS) strain and two drug-resistant strains (diclazuril-resistant (DZR) and maduramicin-resistant (MRR) strains) of Eimeria tenella were carried out by transcriptome sequencing. The expression of glyceraldehyde-3-phosphate dehydrogenase of E. tenella (EtGAPDH) was upregulated in the two resistant strains. In this study, we cloned and characterized EtGAPDH. Indirect immunofluorescence localization was used to observe the distribution of EtGAPDH in E. tenella. The results showed that the protein was distributed mainly on the surface of sporozoites and merozoites, and in the cytoplasm of merozoites. qPCR was performed to detect the transcription level of EtGAPDH in the different developmental stages of the E. tenella DS strain. The transcription level of EtGAPDH was significantly higher in second-generation merozoites than in the other three stages. The transcription level of EtGAPDH in the different drug-resistant strains and DS strain of E. tenella was also analyzed by qPCR. The results showed that the transcription level was significantly higher in the two drug-resistant strains (MRR and DZR) than in the DS strain. As the concentration of diclazuril and maduramicin increased, the transcription levels also increased. Western blot results showed that EtGAPDH protein was upregulated in the DZR and MRR strains. Enzyme activity showed that the enzyme activity of EtGAPDH was higher in the two resistant strains than in the DS strain. These results showed that EtGAPDH possess several roles that separate and distinct from its glycolytic function and maybe involved in the development of E. tenella resistance to anticoccidial drugs.

Keywords: Drug resistance; Eimeria tenella; Glyceraldehyde-3-phosphate dehydrogenase.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Blake DP (2015) Eimeria genomics: where are we now and where are we going? Vet Parasitol 212(1–2):68–74 - PubMed - DOI
    1. Burgess RR (2009) Elution of proteins from gels. Methods Enzymol 463:565–572 - PubMed - DOI
    1. Camacho-Jiménez L, Peregrino-Uriarte AB, Martínez-Quintana JA, Yepiz-Plascencia G (2018) The glyceraldehyde-3-phosphate dehydrogenase of the shrimp Litopenaeus vannamei: molecular cloning, characterization and expression during hypoxia. Mar Environ Res 138:65–75 - PubMed - DOI
    1. Cha SJ, Kim MS, Pandey A, Jacobs-Lorena M (2016) Identification of GAPDH on the surface of Plasmodium sporozoites as a new candidate for targeting malaria liver invasion. J Exp Med 213(10):2099–2112 - PubMed - PMC - DOI
    1. Chapman HD, Jeffers TK (2014) Vaccination of chickens against coccidiosis ameliorates drug resistance in commercial poultry production. Int J Parasitol Drugs Drug Resist 4(3):214–217 - PubMed - PMC - DOI

Substances

LinkOut - more resources