Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live

STAR Protoc. 2022 Mar 30;3(2):101246. doi: 10.1016/j.xpro.2022.101246. eCollection 2022 Jun 17.


Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

Keywords: Cancer; Cell Biology; Cell culture; Microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Combined Modality Therapy
  • Electric Stimulation Therapy* / methods
  • Humans
  • Neoplasms* / diagnostic imaging