Characterization of the complete plastome of Delphinium montanum, a polyploid, endemic and endangered Pyrenean Larkspur

Pascaline Salvado; Christel Llauro; Marie-Christine Carpentier; Valérie Delorme-Hinoux; Joris A M Bertrand

doi:10.1080/23802359.2022.2057248

Characterization of the complete plastome of Delphinium montanum, a polyploid, endemic and endangered Pyrenean Larkspur

Mitochondrial DNA B Resour. 2022 Apr 3;7(4):590-592. doi: 10.1080/23802359.2022.2057248. eCollection 2022.

Authors

Pascaline Salvado¹, Christel Llauro¹, Marie-Christine Carpentier¹, Valérie Delorme-Hinoux¹, Joris A M Bertrand¹

Affiliation

¹ Laboratoire Génome & Développement des Plantes (UMR 5096 UPVD/CNRS), University of Perpignan Via Domitia, Perpignan, France.

Abstract

Delphinium montanum DC. 1815, is an endangered larkspur endemic to the Eastern Pyrenees. For biogeographic and conservation purpose, a hybrid assembly approach based on long- and short-read genomic data allowed us to successfully assemble whole plastid genome of Delphinium montanum. The complete plastome is 154,185 bp in length, consisting of a pair of inverted repeats (IRs) of 26,559 bp, a large single-copy (LSC) region and a small single-copy region (SSC) of 84,746 and 16,320 bp, respectively. It was found to contain 136 genes, including 84 protein-coding genes, 44 trRNA genes and 8 rRNA genes. The overall GC content of the plastid genome is 38.3%. Phylogenetic inference supports the polyphyly of the Delphinium genus.

Keywords: Delphinium montanum; Illumina sequencing; Nanopore sequencing; Pyrenean Larkspur; Ranunculaceae; chloroplast (cp) genome.

Grants and funding

This study was funded by the interreg-POCTEFA program [FLORALAB Project EFA294/19] and is set within the framework of the “Laboratoires d’Excellences (LABEX)” TULIP [ANR-10-LBX-41] as well as within the framework “École Universitaire de Recherche (EUR)” TULIP-GS [ANR-18-EURE-0019].