Der p 1 Disrupts the Epithelial Barrier and Induces IL-6 Production in Patients With House Dust Mite Allergic Rhinitis

Kazuhiro Ogi; Mahnaz Ramezanpour; Sha Liu; Jannatul Ferdoush Tuli; Catherine Bennett; Masanobu Suzuki; Shigeharu Fujieda; Alkis James Psaltis; Peter-John Wormald; Sarah Vreugde

doi:10.3389/falgy.2021.692049

Der p 1 Disrupts the Epithelial Barrier and Induces IL-6 Production in Patients With House Dust Mite Allergic Rhinitis

Front Allergy. 2021 Aug 3:2:692049. doi: 10.3389/falgy.2021.692049. eCollection 2021.

Authors

Kazuhiro Ogi^{1

2

3}, Mahnaz Ramezanpour^{1

2}, Sha Liu^{1

2}, Jannatul Ferdoush Tuli^{1

2}, Catherine Bennett^{1

2}, Masanobu Suzuki^{1

2}, Shigeharu Fujieda³, Alkis James Psaltis^{1

2}, Peter-John Wormald^{1

2}, Sarah Vreugde^{1

2}

Affiliations

¹ Department of Surgery-Otolaryngology, Head and Neck Surgery, University of Adelaide, Adelaide, SA, Australia.
² Central Adelaide Local Health Network, The Queen Elizabeth Hospital, Basil Hetzel Institute for Translational Health Research, Woodville South, SA, Australia.
³ Division of Otorhinolaryngology Head and Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.

Abstract

Background: Dermatophagoides pteronyssinus 1/2 (Der p 1/Der p 2) are regarded as important allergens of house dust mite (HDM). However, the effect of both products on the epithelial barrier and immune response of patients with and without HDM allergic rhinitis (AR) remains unclear. Methods: Air-liquid interface (ALI) cultured human nasal epithelial cells (HNECs) derived from control subjects (non-AR) (n = 9) and HDM-AR patients (n = 9) were treated with Der P 1 and Der P 2, followed by testing the transepithelial electrical resistance (TEER), paracellular permeability of fluorescein isothiocyanate (FITC)-dextrans and immunofluorescence of claudin-1 and ZO-1. Interleukin-6 (IL-6) production was evaluated by ELISA. Results: Der p 1 reduced TEER significantly in a transient and dose-dependent manner in HNEC-ALI cultures from HDM-AR and non-AR patients, whilst the paracellular permeability was not affected. TEER was significantly reduced by Der p 1 at the 10-min time point in HDM-AR patients compared to non-AR patients (p = 0.0259). Compared to no-treatment control, in HNECs derived from HDM-AR patients, Der p 1 significantly cleaved claudin-1 after 30 min exposure (72.7 ± 9.5 % in non-AR group, 39.9 ± 7.1 % in HDM-AR group, p = 0.0286) and induced IL-6 secretion (p = 0.0271). Conclusions: Our results suggest that patients with HDM-AR are more sensitive to Der p 1 than non-AR patients with increased effects of Der p1 on the mucosal barrier and induction of inflammation, indicating an important role for Der p1 in sensitization and HDM-AR development.

Keywords: Der p 1; allergic rhinitis; claudin-1; house dust mite; primary nasal epithelial cells.