Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli

J Bacteriol. 1987 Jan;169(1):26-32. doi: 10.1128/jb.169.1.26-32.1987.

Abstract

Heat and various inhibitory chemicals were tested in Escherichia coli for the ability to cause accumulation of adenylylated nucleotides and to induce proteins of the heat shock (htpR-controlled), the oxidation stress (oxyR-controlled), and the SOS (lexA-controlled) regulons. Under the conditions used, heat and ethanol initiated solely a heat shock response, hydrogen peroxide and 6-amino-7-chloro-5,8-dioxoquinoline (ACDQ) induced primarily an oxidation stress response and secondarily an SOS response, nalidixic acid and puromycin induced primarily an SOS and secondarily a heat shock response, isoleucine restriction induced a poor heat shock response, and CdCl2 strongly induced all three stress responses. ACDQ, CdCl2, and H2O2 each stimulated the synthesis of approximately 35 proteins by factors of 5- to 50-fold, and the heat shock, oxidation stress, and SOS regulons constituted a minor fraction of the overall cellular response. The pattern of accumulation of adenylylated nucleotides during these treatments was inconsistent with a simple role for these nucleotides as alarmones sufficient for triggering the heat shock response, but was consistent with a role in the oxyR-mediated response.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenine Nucleotides / pharmacology
  • Aminoquinolines / pharmacology
  • Cadmium / pharmacology
  • Cadmium Chloride
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Ethanol / pharmacology
  • Gene Expression Regulation / drug effects
  • Guanine Nucleotides / pharmacology
  • Heat-Shock Proteins / genetics*
  • Hydrogen Peroxide / pharmacology
  • Nalidixic Acid / pharmacology
  • Nucleotides / metabolism*
  • Puromycin / pharmacology

Substances

  • Adenine Nucleotides
  • Aminoquinolines
  • Guanine Nucleotides
  • Heat-Shock Proteins
  • Nucleotides
  • Cadmium
  • 6-amino-7-chloro-5,8-dioxoquinoline
  • Nalidixic Acid
  • Ethanol
  • Puromycin
  • Hydrogen Peroxide
  • Cadmium Chloride