Combined RNA-seq and molecular biology technology revealed the protective effect of Cyclocarya paliurus polysaccharide on H2O2-induced oxidative damage in L02 cells thought regulating mitochondrial function, oxidative stress and PI3K/Akt and MAPK signaling pathways

Abstract

Targeting attenuates oxidative damage may serve as a promising strategy for chronic diseases therapy. Cyclocarya paliurus polysaccharide (CP) had shown promising protection effects on L02 cells in oxidative damage. However, its mechanism remains elusive. Here, the protective effect of CP against oxidative damage was demonstrated in an L02 cell line. RNA-seq analysis showed that CP regulated oxidative and mitochondrion-related signal pathways, and the results were further confirmed with experiments. Seahorse extracellular flux experiments indicated that CP recovered mitochondrion function, thereby restoring normal respiration in the cells. In addition, CP regulated the activity of phosphoinositide 3-kinase//protein kinase B (PI3K/Akt) and mitogen-activated protein kinase (MAPKs) and it increased the expression of antioxidant enzymes (SOD, HO-1, and NQO1). Hence, our data suggested that CP protected L02 cells by targeting attenuate oxidative stress and recovering mitochondrion function, which might be achieved through PI3K/Akt and MAPK signaling. In summary, CP may be used as a functional food and may alleviate chronic diseases involving oxidative damage.

Keywords: Cyclocarya paliurus; Mitochondrion function; Oxidative damage; Polysaccharide; RNA-seq; Signaling pathways.