Comparative esterase electrophoretic polymorphism of Escherichia coli isolates obtained from animal and human sources

J Gen Microbiol. 1986 Jul;132(7):1843-51. doi: 10.1099/00221287-132-7-1843.

Abstract

To determine whether enzyme electrophoretic polymorphism in Escherichia coli populations was influenced by environmental background, the mobilities of four electrophoretically variable esterases (A, B, C and I) were examined. The distinction between isolates was established by significant differences in the electrophoretic distribution and the genetic diversity coefficient of individual esterases. Principal components analysis on each population and on all strains revealed three groups of allozymes. The first, characterized by slow electrophoretic mobilities of esterase B, was frequently observed in strains obtained from human extra-intestinal infections and rarely in commensal organisms. The second, characterized by fast mobilities of esterases A and B, was frequently found in animal isolates. The third, characterized by prominence of the most common mobilities of esterases B and A, was recovered in all populations. These results were confirmed by discriminant analysis. Among the 610 strains investigated, 316 electrophoretic types (distinctive combinations of allozymes of the four varieties of esterases) were distinguished, illustrating high esterase polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carboxylesterase
  • Carboxylic Ester Hydrolases / classification
  • Cattle
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Esterases / classification
  • Esterases / genetics*
  • Genetic Variation
  • Humans
  • Male
  • Naphthol AS D Esterase / metabolism
  • Polymorphism, Genetic*
  • Rabbits
  • Rats

Substances

  • Esterases
  • Naphthol AS D Esterase
  • arginine esterase
  • Carboxylic Ester Hydrolases
  • Carboxylesterase