Identification and charactering of APX genes provide new insights in abiotic stresses response in Brassica napus

Jiao Pan; Lei Zhang; Min Chen; Yuxuan Ruan; Peifang Li; Zhihui Guo; Boyu Liu; Ying Ruan; Mu Xiao; Yong Huang

doi:10.7717/peerj.13166

Identification and charactering of APX genes provide new insights in abiotic stresses response in Brassica napus

PeerJ. 2022 Apr 5:10:e13166. doi: 10.7717/peerj.13166. eCollection 2022.

Authors

Jiao Pan^{1

2}, Lei Zhang^{1

2}, Min Chen^{1

2}, Yuxuan Ruan^{1

2}, Peifang Li^{1

2}, Zhihui Guo^{1

2}, Boyu Liu^{1

2}, Ying Ruan^{1

2}, Mu Xiao^{1

2}, Yong Huang^{1

2}

Affiliations

¹ College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, China.
² Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, Changsha, Hunan, China.

Abstract

Ascorbate peroxidase (APX) plays an important role in scavenging H₂O₂ and balancing ROS content in plant cells, which is of great significance for the growth and development of life and resistance to external stress. However, up to now, APXs in Brassica napus (B. napus) have not been systematically characterized. In this study, a total of 26 BnaAPX genes were identified, which were distributed on 13 chromosomes and divided into five phylogenetic branches. Gene structure analysis showed that they had a wide varied number of exons while BnaAPXs proteins contained more similar motifs in the same phylogenetic branches. qRT-PCR analysis of 26 BnaAPX gene expression patterns showed that three putative cytosol BnaAPX genes BnaAPX1, BnaAPX2, BnaAPX9, two putatice microsomal genes BnaAPX18 and BnaAPX25 were up-regulated rapidly and robustly under high salt, water shortage and high temperature stresses. In addition, the above three abiotic stresses led to a significant increase in APX activity. The results provide basic and comprehensive information for further functional characterization of APX gene family in B. napus.

Keywords: Abiotic stress; Ascorbate peroxidase; Brassica napus; Expression pattern.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascorbate Peroxidases / genetics
Brassica napus* / genetics
Hydrogen Peroxide
Phylogeny
Stress, Physiological / genetics

Substances

Ascorbate Peroxidases
Hydrogen Peroxide

Grants and funding

This work was funded by National Nature Science Foundation of China (31971834), Natural Science Foundation of Hunan Province (2019JJ40116). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.