Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells

Tongjie Wang; Cui Lv; Fangxiao Hu; Lijuan Liu; Jinyong Wang

doi:10.1097/BS9.0000000000000049

Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells

Blood Sci. 2020 Jul 25;2(3):79-88. doi: 10.1097/BS9.0000000000000049. eCollection 2020 Jul.

Authors

Tongjie Wang^{1

2}, Cui Lv^{1

2}, Fangxiao Hu^{1

2}, Lijuan Liu^{1

2}, Jinyong Wang^{1

2

3}

Affiliations

¹ State Key Laboratory of Experimental Hematology, CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
² Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, 510530, Guangzhou, China.
³ Bioisland Laboratory, 510005, Guangzhou, China.

Abstract

Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible Runx1-Hoxa9-PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.

Keywords: Induced hematopoietic progenitor cells; Pluripotent stem cells; T cells regeneration; Transplantation.