Patient-Derived Xenotransplant of CNS Neoplasms in Zebrafish: A Systematic Review

Cells. 2022 Apr 2;11(7):1204. doi: 10.3390/cells11071204.


Glioblastoma and neuroblastoma are the most common central nervous system malignant tumors in adult and pediatric populations. Both are associated with poor survival. These tumors are highly heterogeneous, having complex interactions among different cells within the tumor and with the tumor microenvironment. One of the main challenges in the neuro-oncology field is achieving optimal conditions to evaluate a tumor's molecular genotype and phenotype. In this respect, the zebrafish biological model is becoming an excellent alternative for studying carcinogenic processes and discovering new treatments. This review aimed to describe the results of xenotransplantation of patient-derived CNS tumors in zebrafish models. The reviewed studies show that it is possible to maintain glioblastoma and neuroblastoma primary cell cultures and transplant the cells into zebrafish embryos. The zebrafish is a suitable biological model for understanding tumor progression and the effects of different treatments. This model offers new perspectives in providing personalized care and improving outcomes for patients living with central nervous system tumors.

Keywords: CNS neoplasms; glioblastoma; neuroblastoma; patient-derived; xenotransplant; zebrafish.

Publication types

  • Review
  • Systematic Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System Neoplasms*
  • Glioblastoma* / pathology
  • Humans
  • Neuroblastoma* / genetics
  • Tumor Microenvironment
  • Zebrafish / genetics