Localizing Total mRNA in Plant Cells

Geraint Parry

doi:10.1007/978-1-0716-2337-4_7

Localizing Total mRNA in Plant Cells

Methods Mol Biol. 2022:2502:105-111. doi: 10.1007/978-1-0716-2337-4_7.

Author

Geraint Parry¹

Affiliation

¹ GARNet, School of Biosciences, Cardiff University, Cardiff, UK. arabidopsiseventsuk@gmail.com.

PMID: 35412234
DOI: 10.1007/978-1-0716-2337-4_7

Abstract

Visualizing the location of the total cellular mRNA pool can be important to understand how different genes affect cellular physiology. Over the past decade researchers investigating RNA processing, nuclear transport and the function of the nuclear pore complex have used in situ hybridization protocol to visualize and quantify the accumulation of the total mRNA pool within the plant cell nucleus.

Keywords: In situ hybridization; Nuclear pore complex; Nuclear transport; Nucleus; mRNA.

MeSH terms

Active Transport, Cell Nucleus / physiology
Cell Nucleus / genetics
Cell Nucleus / metabolism
Nuclear Pore / metabolism
Nuclear Pore Complex Proteins* / metabolism
Plant Cells* / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

Nuclear Pore Complex Proteins
RNA, Messenger