Advanced Glycation End Products Promote Melanogenesis by Activating NLRP3 Inflammasome in Human Dermal Fibroblasts

J Invest Dermatol. 2022 Oct;142(10):2591-2602.e8. doi: 10.1016/j.jid.2022.03.025. Epub 2022 Apr 11.


Advanced glycation end product (AGE) accumulation is significantly increased in the dermis of photoaged skin and plays crucial roles in photoaging. Although AGEs have been found to contribute to the yellowish discoloration of photoaged skin, their roles in photoaging-associated hyperpigmentation disorders have not been extensively studied. In this study, we observed that AGEs, NLRP3, and IL-18 were increased in the dermis of sun-exposed skin and lesions of melasma and solar lentigo and that dermal deposition of AGE was positively correlated with epidermal melanin levels. In addition, we found that AGE-BSA potently activated NLRP3 inflammasome and promoted IL-18 production and secretion in cultured fibroblasts, which was mediated by receptor for AGE/NF-κB pathway. Moreover, AGE-BSA significantly promoted melanogenesis by increasing tyrosinase activity and expression of microphthalmia-associated transcription factor and tyrosinase, which was dependent on NLRP3 inflammasome activation and IL-18 secretion in fibroblasts. Notably, AGE-collagen could activate NLRP3 inflammasome in fibroblasts and enhance melanogenesis. Furthermore, we found that IL-18 enhanced melanogenesis by binding to its receptor and activating p38 MAPK and extracellular signal‒regulated kinase 1/2 signaling pathways in melanocytes. Importantly, the promelanogenesis of AGE-BSA was verified in ex vivo cultured skin and mouse models. These findings suggest that dermal AGEs stimulate melanogenesis and contribute to the development of photoaging-associated hyperpigmentation disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fibroblasts / metabolism
  • Glycation End Products, Advanced / metabolism
  • Humans
  • Inflammasomes* / metabolism
  • Interleukin-18 / metabolism
  • Lentigo*
  • Melanins / metabolism
  • Mice
  • Microphthalmia-Associated Transcription Factor / metabolism
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Monophenol Monooxygenase / metabolism
  • NF-kappa B / metabolism
  • NLR Family, Pyrin Domain-Containing 3 Protein / metabolism
  • p38 Mitogen-Activated Protein Kinases / metabolism


  • Glycation End Products, Advanced
  • Inflammasomes
  • Interleukin-18
  • Melanins
  • Microphthalmia-Associated Transcription Factor
  • NF-kappa B
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Nlrp3 protein, mouse
  • Monophenol Monooxygenase
  • Mitogen-Activated Protein Kinase 3
  • p38 Mitogen-Activated Protein Kinases