Identification of a possible nucleotide binding site in CheW, a protein required for sensory transduction in bacterial chemotaxis

J Biol Chem. 1987 Jan 15;262(2):535-7.

Abstract

CheW is an essential component of the system which mediates chemotaxis in Salmonella typhimurium and Escherichia coli. Here we report the nucleotide sequence of the cheW gene as well as the purification and characterization of the CheW protein. The DNA sequence predicts a protein of 18,000 molecular weight. The pure protein exhibits an apparent molecular weight of 18,000 during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Molecular sieve chromatography under nondenaturing conditions indicates a molecular weight of approximately 35,000, however. This result suggests that CheW is a homodimer. The predicted amino acid sequence between Thr-128 and Asp-160 fits a consensus exhibited by many proteins which bind purine nucleotides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Base Sequence
  • Binding Sites
  • Escherichia coli Proteins*
  • Genes*
  • Genes, Bacterial*
  • Molecular Weight
  • Nucleotides / metabolism
  • Plasmids
  • Protein Binding
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / metabolism

Substances

  • Bacterial Proteins
  • CheW protein, E coli
  • Escherichia coli Proteins
  • Nucleotides
  • CheW protein, Bacteria

Associated data

  • GENBANK/J02656