Differentiation of rat bone marrow cells into macrophages under the influence of mouse L929 cell supernatant

J Leukoc Biol. 1987 Jan;41(1):83-91. doi: 10.1002/jlb.41.1.83.

Abstract

Bone marrow cells (BMC) flushed from femora of Lewis rats were cultured in Dulbecco's modification of Eagle's medium supplemented with mouse L929 cell supernatant as a source of colony-stimulating factor (CSF). Differentiation of macrophage progenitor cells into macrophages (M phi) and expression of various markers were kinetically assessed. The proportion of M phi increases from approximately 4% in freshly isolated BMC to 100% after 7-8 days of cell culture. These cells, termed bone marrow cell-derived macrophages (BMDM phi), adhere to and spread on plastic surface; exhibit M phi morphology; stain intensely for nonspecific esterase; are able to phagocytose latex particles, IgG-sensitized erythrocytes, and C3-coated red cells; and express receptors for IgG and C3. A subpopulation of BMDM phi expresses MHC class II antigens as demonstrated by immunofluorescence using MRC OX6 and MRC OX17 monoclonal antibodies which recognize antigens coded in the I-A or I-E subregion of the MHC, respectively. Collectively, our results show that supernatant from mouse L929 cells supports and is continuously required for proliferation and differentiation of rat BMC into typical M phi, and suggest that mouse CSF cross-reacts with the putative receptor on rat M phi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells*
  • Cell Differentiation
  • Cell Division
  • Colony-Stimulating Factors / pharmacology*
  • Histocompatibility Antigens Class II / analysis
  • Histocytochemistry
  • Macrophage-1 Antigen
  • Macrophages / cytology*
  • Macrophages / immunology
  • Male
  • Mice
  • Phagocytosis
  • Rats
  • Rats, Inbred Lew
  • Receptors, Complement / analysis
  • Receptors, Fc / analysis
  • Species Specificity

Substances

  • Colony-Stimulating Factors
  • Histocompatibility Antigens Class II
  • Macrophage-1 Antigen
  • Receptors, Complement
  • Receptors, Fc