Rapid FISH results within one hour

Cancer Genet. 2022 Jun:264-265:66-70. doi: 10.1016/j.cancergen.2022.03.005. Epub 2022 Apr 8.


This study evaluated a rapid fluorescence in-situ hybridization (FISH) method with a novel 10 min hybridization time to identify the presence of recurrent gene re-arrangements in patients with Haematological malignancies (HMs). A method comparison experimental approach was used to compare this rapid method to the standard method. Hybridization results using the rapid method were comparable to standard methods in terms of result, signal strength and hybridization quality. This rapid FISH turn around time (TAT) was 1 h and enabled same day reporting of genetic and morphology results for a prompt diagnosis and timely access to targeted therapies.

Keywords: FISH; Haematological malignancies; Intellifish; Leukaemia diagnosis; Rapid test.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Hematologic Neoplasms* / diagnosis
  • Hematologic Neoplasms* / genetics
  • Humans
  • In Situ Hybridization, Fluorescence / methods