Phosphate dysregulation via the XPR1-KIDINS220 protein complex is a therapeutic vulnerability in ovarian cancer

Nat Cancer. 2022 Jun;3(6):681-695. doi: 10.1038/s43018-022-00360-7. Epub 2022 Apr 18.


Despite advances in precision medicine, the clinical prospects for patients with ovarian and uterine cancers have not substantially improved. Here, we analyzed genome-scale CRISPR-Cas9 loss-of-function screens across 851 human cancer cell lines and found that frequent overexpression of SLC34A2-encoding a phosphate importer-is correlated with sensitivity to loss of the phosphate exporter XPR1, both in vitro and in vivo. In patient-derived tumor samples, we observed frequent PAX8-dependent overexpression of SLC34A2, XPR1 copy number amplifications and XPR1 messenger RNA overexpression. Mechanistically, in SLC34A2-high cancer cell lines, genetic or pharmacologic inhibition of XPR1-dependent phosphate efflux leads to the toxic accumulation of intracellular phosphate. Finally, we show that XPR1 requires the novel partner protein KIDINS220 for proper cellular localization and activity, and that disruption of this protein complex results in acidic "vacuolar" structures preceding cell death. These data point to the XPR1-KIDINS220 complex and phosphate dysregulation as a therapeutic vulnerability in ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Female
  • Humans
  • Membrane Proteins* / genetics
  • Membrane Proteins* / metabolism
  • Nerve Tissue Proteins* / genetics
  • Nerve Tissue Proteins* / metabolism
  • Ovarian Neoplasms* / drug therapy
  • Ovarian Neoplasms* / genetics
  • Ovarian Neoplasms* / metabolism
  • Phosphates / pharmacology
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, Virus / genetics
  • Xenotropic and Polytropic Retrovirus Receptor / genetics
  • Xenotropic and Polytropic Retrovirus Receptor / metabolism


  • KIDINS220 protein, human
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Phosphates
  • Receptors, G-Protein-Coupled
  • Receptors, Virus
  • XPR1 protein, human
  • Xenotropic and Polytropic Retrovirus Receptor

Associated data

  • figshare/10.6084/m9.figshare.19125671.v1
  • figshare/10.6084/m9.figshare.19125677.v1