LncRNA PVT1 is increased in renal cell carcinoma and affects viability and migration in vitro

J Clin Lab Anal. 2022 Jun;36(6):e24442. doi: 10.1002/jcla.24442. Epub 2022 Apr 20.


Background: Renal cell carcinoma is difficult to diagnose and unpredictable in disease course and severity. There are no specific biomarkers for diagnosis and prognosis estimation feasible in clinical practice. Long non-coding RNAs (lncRNAs) have emerged as potent regulators of gene expression in recent years. Aside from their cellular role, their expression patterns could be used as a biomarker of ongoing pathology.

Methods: In this work, we used next-generation sequencing for global lncRNA expression profiling in tumor and non-tumor tissue of RCC patients. The four candidate lncRNAs have been further validated on an independent cohort. PVT1, as the most promising lncRNA, has also been studied using functional in vitro tests.

Results: Next-generation sequencing showed significant dysregulation of 1163 lncRNAs; among them top 20 dysregulated lncRNAs were AC061975.7, AC124017.1, AP000696.1, AC148477.4, LINC02437, GATA3-AS, LINC01762, LINC01230, LINC01271, LINC01187, LINC00472, AC007849.1, LINC00982, LINC01543, AL031710.1, and AC019197.1 as down-regulated lncRNAs; and SLC16A1-AS1, PVT1, LINC0887, and LUCAT1 as up-regulated lncRNAs. We observed statistically significant dysregulation of PVT1, LUCAT1, and LINC00982. Moreover, we studied the effect of artificial PVT1 decrease in renal cell line 786-0 and observed an effect on cell viability and migration.

Conclusion: Our results show not only the diagnostic but also the therapeutic potential of PVT1 in renal cell carcinoma.

Keywords: diagnosis; long non-coding RNA; migration next-generation sequencing; proliferation; transcriptome.

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / physiology
  • Carcinoma, Renal Cell* / genetics
  • Carcinoma, Renal Cell* / physiopathology
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Cell Survival / genetics
  • Gene Expression Regulation, Neoplastic
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Kidney Neoplasms* / genetics
  • Kidney Neoplasms* / physiopathology
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • RNA, Long Noncoding* / physiology


  • Biomarkers, Tumor
  • PVT1 long-non-coding RNA, human
  • RNA, Long Noncoding
  • long non-coding RNA LUCAT1, human