Role of fluorescence imaging for intraoperative intestinal assessment in gynecological surgery: a systematic review

Minim Invasive Ther Allied Technol. 2022 Oct;31(7):992-999. doi: 10.1080/13645706.2022.2064715. Epub 2022 Apr 20.


Background: Our aim was to review the current knowledge of the role of fluorescence imaging for intraoperative intestinal assessment in gynecological surgery.

Material and methods: A computer-based systematic review was performed from 2000 to 2020. All articles describing the use of indocyanine green (ICG) applied to bowel assessments in gynecology or endometriosis surgery were considered for review.

Results: ICG is an effective tool for assessing bowel vascularization, potentially preventing anastomotic leakage and recto-vaginal fistula and can therefore be useful for endometriosis surgery or bowel assessment in gynecological oncology procedures. Real-time characterization of the hypovascular pattern of endometriotic nodules has been associated with a larger nodule size and lower microvessel density, helping surgeons choose the best transecting line and the most appropriate technique. ICG angiography allows for a laparoscopic and intrarectal bowel assessment, which can act as a double check of bowel perfusion, enabling the assessment of mucosa vascularization. ICG fluorescence can guide intraoperative decision-making after intestinal anastomosis, discoid resection, and rectal shaving, preventing anastomotic leakage and postoperative recto-vaginal fistula in low anterior resections.

Conclusions: ICG angiography provides a better intestinal assessment. Larger, prospective, randomized controlled studies are needed to validate the technique and confirm these encouraging results.

Keywords: Minimally invasive surgery; bowel complication; gynecological surgery; indocyanine green.

Publication types

  • Review
  • Systematic Review

MeSH terms

  • Anastomosis, Surgical / methods
  • Anastomotic Leak / prevention & control
  • Anastomotic Leak / surgery
  • Endometriosis* / surgery
  • Female
  • Gynecologic Surgical Procedures
  • Humans
  • Indocyanine Green
  • Laparoscopy* / methods
  • Optical Imaging / methods
  • Prospective Studies
  • Vaginal Fistula* / surgery


  • Indocyanine Green