Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis

Fangze Guo; Changqing Yuan; Hailin Huang; Xuyang Deng; Zirui Bian; Danyang Wang; Keke Dou; Li Mei; Qihui Zhou

doi:10.3389/fbioe.2022.884291

Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis

Front Bioeng Biotechnol. 2022 Apr 4:10:884291. doi: 10.3389/fbioe.2022.884291. eCollection 2022.

Authors

Fangze Guo^{1

2}, Changqing Yuan^{1

2}, Hailin Huang^{1

2}, Xuyang Deng^{1

2}, Zirui Bian², Danyang Wang^{1

2}, Keke Dou^{1

2}, Li Mei^{1

2

3}, Qihui Zhou^{1

2

3}

Affiliations

¹ Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China.
² School of Stomatology, Qingdao University, Qingdao, China.
³ Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China.

Abstract

Nano-hydroxyapatite (nHA) has been widely applied as a tissue-engineering biomaterial and interacted with osteoblasts/stem cells to repair bone defects. In addition, T cells that coexist with osteoblasts/stem cells in the bone modulate the regulation of osteoimmunology by cytokine formation. However, the effects of nHA on T cells and the following regulatory interplay on osteogenic differentiation have been rarely examined. In this work, the physicochemical properties of needle-like nHA are characterized by field emission scanning electron microscopy, zeta potential, Fourier transform-infrared and X-ray diffraction. It is found that as the concentration of nHA increases, the proliferation of T cells gradually increases, and the proportion of apoptotic T cells decreases. The percentage of CD4⁺ T cells is higher than that of CD8⁺ T cells under the regulation of needle-like nHA. Furthermore, the supernatant of T cells co-cultured with nHA significantly inhibits the osteogenic differentiation of MC3T3-E1 by downregulating the formation of alkaline phosphatase and calcium nodule compared with the supernatant of nHA. Thus, our findings provide new insight into the nHA-mediated T cell and osteoblast interactions.

Keywords: T cell; nano-hydroxyapatite; nanomaterials; osteogenesis; osteoimmunology.