Development and Application of CRISPR-Cas Based Tools

Yanping Hu; Wei Li

doi:10.3389/fcell.2022.834646

Development and Application of CRISPR-Cas Based Tools

Front Cell Dev Biol. 2022 Apr 4:10:834646. doi: 10.3389/fcell.2022.834646. eCollection 2022.

Authors

Yanping Hu^{1

2

3}, Wei Li^{1

2

3

4

5}

Affiliations

¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
² University of Chinese Academy of Sciences, Beijing, China.
³ Institute for Stem Cell and Regenerative Medicine, Chinese Academy of Sciences, Beijing, China.
⁴ Bejing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
⁵ HIT Center for Life Sciences, Harbin Institute of Technology, Harbin, China.

Abstract

Abundant CRISPR-Cas systems in nature provide us with unlimited valuable resources to develop a variety of versatile tools, which are powerful weapons in biological discovery and disease treatment. Here, we systematically review the development of CRISPR-Cas based tools from DNA nuclease to RNA nuclease, from nuclease dependent-tools to nucleic acid recognition dependent-tools. Also, considering the limitations and challenges of current CRISPR-Cas based tools, we discuss the potential directions for development of novel CRISPR toolkits in the future.

Keywords: CRISPR toolkits; Cas12; Cas13; Cas9; gene editing.

Publication types

Review