Chlorinated metabolites with antibacterial activities from a deep-sea-derived Spiromastix fungus

Siwen Niu; Dong Liu; Zongze Shao; Jiang Huang; Aili Fan; Wenhan Lin

doi:10.1039/d1ra05736g

Chlorinated metabolites with antibacterial activities from a deep-sea-derived Spiromastix fungus

RSC Adv. 2021 Sep 3;11(47):29661-29667. doi: 10.1039/d1ra05736g. eCollection 2021 Sep 1.

Authors

Siwen Niu^{1

2}, Dong Liu¹, Zongze Shao², Jiang Huang¹, Aili Fan¹, Wenhan Lin^{1

3}

Affiliations

¹ State Key Laboratory of Natural and Biomimetic Drugs, Peking University Beijing 100191 P. R. China whlin@bjmu.edu.cn.
² Third Institute of Oceanography, SOA Xiamen 361005 P. R. China.
³ Institute of Ocean Research, Ningbo Institute of Marine Medicine, Peking University Beijing 100191 P. R. China.

Abstract

Chromatographic separation of the solid cultures of a deep-sea-derived Spiromastix fungus (MCCC 3A00308) resulted in the isolation of eight compounds. Their structures were identified on the basis of the spectroscopic data. Compounds 1-8 are classified as depsidone-type (1-4), isocoumarin-type (5 and 6), and benzothiazole-type (7 and 8), of which 1-7 are new compounds and 1-3 along with 5 and 6 are chlorinated. Compound 3 is characterized by trichlorination and shows potent activities against Gram-positive pathogenic bacteria including Staphylococcus aureus ATCC 25923, Bacillus thuringiensis ATCC 10792, and Bacillus subtilis CMCC 63501, with minimum inhibitory concentration (MIC) values ranging from 0.5 to 1.0 μg mL^-1. This study extends the chemical diversity of chlorinated natural products from marine-derived fungi and provides a promising lead for the development of antibacterial agents.