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. 2021 Jul 5;11(38):23627-23630.
doi: 10.1039/d1ra03472c. eCollection 2021 Jul 1.

Absolute content determination by quantitative NMR (qNMR) spectroscopy: a curious case of aldosterone

Neeraj Singh  1 , Judith Taibon  1 , Stephan Pongratz  1 , Christian Geletneky  1
Affiliations

Absolute content determination by quantitative NMR (qNMR) spectroscopy: a curious case of aldosterone

Neeraj Singh et al. RSC Adv. .

Abstract

Quantitative NMR spectroscopy has been utilized to calculate the absolute content (g g-1) of aldosterone, which is necessary for electrolyte balance and blood pressure regulation, in commercially available materials. Explanations have been provided for many signals observed in the 1HNMR spectrum, false interpretation of which can have significant effects if such a value is utilized for the primary calibrators in ID-LC-MS/MS ('gold standard') reference methods in clinical chemistry.

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Conflict of interest statement

All the authors are employees of Roche Diagnostics, Nonnenwald 2, 82377 Penzberg, Germany. There are no conflicts to declare.

Figures

Scheme 1
Scheme 1. Structures of aldosterone tautomers and some impurities.
Fig. 1
Fig. 1. Overlay of 1H{13C}NMR spectra of aldosterone 1 (S1: red; S2: blue) indicating the various diastereomers 2a, 2b, 3a, 3b along with the 2D-gHSQCAD spectrum (S1 material).
See this image and copyright information in PMC

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