Enzymatic dephosphorylation-triggered self-assembly of DNA amphiphiles

Jianmei Zou; Qian Gao; Jinfang Nie; Yun Zhang; Cheng Jin

doi:10.1039/d1ra01668g

Enzymatic dephosphorylation-triggered self-assembly of DNA amphiphiles

RSC Adv. 2021 May 21;11(30):18322-18325. doi: 10.1039/d1ra01668g. eCollection 2021 May 19.

Authors

Jianmei Zou^{1

2}, Qian Gao¹, Jinfang Nie¹, Yun Zhang¹, Cheng Jin³

Affiliations

¹ Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology 12 Jiangan Road Guilin 541004 China 2019136@glut.edu.cn.
² Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, Aptamer Engineering Center of Hunan Province, Hunan University Changsha Hunan 410082 China.
³ Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200240 China jinch@hnu.edu.cn.

Abstract

In this work, phosphorylated lipid-conjugated oligonucleotide (DNA-lipid-P) has been synthesized to develop an enzyme-responsive self-assembly of DNA amphiphiles based on dephosphorylation-induced increase of hydrophobicity. Since elevated ALP level is a critical index in some diseases, ALP-triggered self-assembly of DNA amphiphiles shows promise in disease diagnosis and cancer treatment.