Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides

Biochem Biophys Res Commun. 1987 Feb 13;142(3):710-6. doi: 10.1016/0006-291x(87)91472-0.


The "polymerase chain reaction" (PCR) procedure for amplifying specific gene sequences has recently been combined with sequence-specific oligonucleotide (SSO) probe hybridization to develop a highly sensitive, rapid, and simple method for analyzing allelic variations in genomic DNA. In the present study we have used PCR/SSO to analyze partially purified DNA extracted from formalin-fixed, paraffin-embedded tissue specimens. We report that this DNA, including samples that were partially degraded, proved to be suitable for analysis by the PCR/SSO procedure.

MeSH terms

  • Base Sequence
  • DNA / genetics*
  • DNA / isolation & purification
  • DNA-Directed DNA Polymerase*
  • Fixatives
  • Formaldehyde
  • Genotype
  • Globins / genetics*
  • Histological Techniques
  • Humans
  • Nucleic Acid Hybridization*
  • Oligonucleotides*
  • Paraffin


  • Fixatives
  • Oligonucleotides
  • Formaldehyde
  • Paraffin
  • Globins
  • DNA
  • DNA-Directed DNA Polymerase