It's a DoG-eat-DoG world-altered transcriptional mechanisms drive downstream-of-gene (DoG) transcript production

Mol Cell. 2022 Jun 2;82(11):1981-1991. doi: 10.1016/j.molcel.2022.04.008. Epub 2022 Apr 28.


The past decade has revolutionized our understanding of regulatory noncoding RNAs (ncRNAs). Among the most recently identified ncRNAs are downstream-of-gene (DoG)-containing transcripts that are produced by widespread transcriptional readthrough. The discovery of DoGs has set the stage for future studies to address many unanswered questions regarding the mechanisms that promote readthrough transcription, RNA processing, and the cellular functions of the unique transcripts. In this review, we summarize current findings regarding the biogenesis, function, and mechanisms regulating this exciting new class of RNA molecules.

Keywords: CRISPR; elongation; gene expression; next-generation sequencing analysis; noncoding RNAs; readthrough transcription; termination; transcriptional regulation; transcriptomics.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • RNA Processing, Post-Transcriptional
  • RNA, Untranslated* / genetics
  • Transcription, Genetic*


  • RNA, Untranslated