Insulin stimulation of iodothyronine 5'-deiodinase in rat brown adipocytes

Biochem Biophys Res Commun. 1987 Feb 27;143(1):81-6. doi: 10.1016/0006-291x(87)90632-2.

Abstract

Insulin (100-3333 microU/ml) stimulates iodothyronine 5'-deiodinase 3 to 4 fold in dispersed rat brown adipocytes. Deiodinase activity increased steadily from 1 to 4 hours. Insulin increased enzyme activity via an increase in the Vmax while the Km remained unchanged. Omission of glucose from the medium did not affect the insulin response. Studies with alpha-amanitin suggested that the increase in deiodinase activity was not due to an increase in the rate of transcription. The insulin effect was not additive to that of alpha 1-catecholamines, suggesting the two stimulators might have one or more common elements.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adipose Tissue, Brown / drug effects
  • Adipose Tissue, Brown / enzymology*
  • Animals
  • Cells, Cultured
  • Glucose / pharmacology
  • Insulin / pharmacology*
  • Iodide Peroxidase / metabolism*
  • Kinetics
  • Male
  • Norepinephrine / pharmacology
  • Rats
  • Rats, Inbred Strains

Substances

  • Insulin
  • Iodide Peroxidase
  • Glucose
  • Norepinephrine