Anticancer Agents Derived from Cyclic Thiosulfonates: Structure-Reactivity and Structure-Activity Relationships

ChemMedChem. 2022 Jul 19;17(14):e202200165. doi: 10.1002/cmdc.202200165. Epub 2022 May 23.

Abstract

Reported are structure-property-function relationships associated with a class of cyclic thiosulfonate molecules-disulfide-bond disrupting agents (DDAs)-with the ability to downregulate the Epidermal Growth Factor Receptor (HER) family in parallel and selectively induce apoptosis of EGFR+ or HER2+ breast cancer cells. Recent findings have revealed that the DDA mechanism of action involves covalent binding to the thiol(ate) from the active site cysteine residue of members of the protein disulfide isomerase (PDI) family. Reported is how structural modifications to the pharmacophore can alter the anticancer activity of cyclic thiosulfonates by tuning the dynamics of thiol-thiosulfonate exchange reactions, and the studies reveal a correlation between the biological potency and thiol-reactivity. Specificity of the cyclic thiosulfonate ring-opening reaction by a nucleophilic attack can be modulated by substituent addition to a parent scaffold. Lead compound optimization efforts are also reported, and have resulted in a considerable decrease of the IC50 /IC90 values toward HER-family overexpressing breast cancer cells.

Keywords: Anticancer; Breast cancer; Cyclic thiosulfonate; Protein disulfide isomerase inhibitors; Thiol reactivity; Thiosulfonate; Tunable ring opening.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antineoplastic Agents* / pharmacology
  • Cysteine
  • Protein Disulfide-Isomerases
  • Structure-Activity Relationship
  • Sulfhydryl Compounds / chemistry

Substances

  • Antineoplastic Agents
  • Sulfhydryl Compounds
  • Protein Disulfide-Isomerases
  • Cysteine