Effect of co-administration of metformin and extracts of Costus pictus D.Don leaves on alloxan-induced diabetes in rats

Ami Naik; Sherif Babatunde Adeyemi; Bhavin Vyas; Ramar Krishnamurthy

doi:10.1016/j.jtcme.2021.08.007

Effect of co-administration of metformin and extracts of Costus pictus D.Don leaves on alloxan-induced diabetes in rats

J Tradit Complement Med. 2021 Aug 12;12(3):269-280. doi: 10.1016/j.jtcme.2021.08.007. eCollection 2022 May.

Authors

Ami Naik¹, Sherif Babatunde Adeyemi^{1

2}, Bhavin Vyas³, Ramar Krishnamurthy¹

Affiliations

¹ C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli-Mahuva Road, Pin-394350, Surat Dist., Gujarat State, India.
² Ethnobotany Unit, Department of Life Sciences, Faculty of Life Sciences, University of Ilorin, PMB 1515, Ilorin, Kwara State, Nigeria.
³ Maliba Pharmacy College, Uka Tarsadia University, Bardoli-Mahuva Road, Gujarat State, India.

Abstract

Background and aim: The present study evaluates the antidiabetic effects of aqueous (CPAQ) and methanolic (CPME) extract of Costus pictus D. Don singly and/or in combination with metformin in alloxan-induced diabetic rats.

Experimental procedure: CPAQ and CPME (400 mg/kg dose), metformin (120 mg/kg) and two different combinations of plant extracts and metformin (200 + 60 mg/kg and 400 mg/kg + 60 mg/kg) were orally given to alloxan-induced diabetic rats for 21 days. At 0, 7, 14, and 21 days, body weight and blood glucose levels were measured.

Results and conclusion: After 21 days of treatment, biochemical profiling and histopathology analysis were carried out. CPAQ and CPME, when administrated separately, could decrease blood glucose levels (P ≤ 0.05). CPME showed more promising results (P ≤ 0.05) compared to the diabetic control group. Extracts co-administrated with metformin showed dose-dependent significant recovery of hypoglycemic activity of metformin. Fasting blood glucose levels, body weight, protein, and lipid profile of the treatment group were compared to the diabetic and normal control groups. Animal groups co-administered with CPME and metformin showed more significant effects on the recovery of tissue damages. The synergistic effect of plant extracts with metformin has positive effects on all the parameters and enhanced the efficiency and reduction of blood glucose levels.

Keywords: Costaceae; Histopathology; Hypoglycaemia; Insulin; Synergistic.