The nucleotide sequence of the pepN gene and its over-expression in Escherichia coli

Gene. 1986;48(1):145-53. doi: 10.1016/0378-1119(86)90360-4.

Abstract

The complete nucleotide sequence has been determined for the pepN gene of Escherichia coli K-12. The product of this gene, peptidase N, is apparently 870 amino acids in length. The coding sequence is followed by a tandem pair of stop codons and then a sequence capable of forming a stem-and-loop structure in the pepN mRNA. In the process of subcloning the pepN gene we constructed a plasmid which causes peptidase N to be produced at a level of 50% of total protein. The peptidase is fully active and completely soluble and these overproducing cells appear otherwise normal.

MeSH terms

  • Aminopeptidases / biosynthesis
  • Aminopeptidases / genetics*
  • Base Sequence
  • Codon / genetics
  • DNA, Bacterial / genetics*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Gene Expression Regulation
  • Genes
  • Plasmids
  • Promoter Regions, Genetic

Substances

  • Codon
  • DNA, Bacterial
  • Aminopeptidases
  • peptidase N

Associated data

  • GENBANK/M15273