An in vivo orthotopic serial passaging model for a metastatic renal cancer study

Jun Nishida; Kosuke Miyakuni; Kohei Miyazono; Shogo Ehata

doi:10.1016/j.xpro.2022.101306

An in vivo orthotopic serial passaging model for a metastatic renal cancer study

STAR Protoc. 2022 Apr 18;3(2):101306. doi: 10.1016/j.xpro.2022.101306. eCollection 2022 Jun 17.

Authors

Jun Nishida¹, Kosuke Miyakuni¹, Kohei Miyazono¹, Shogo Ehata¹

Affiliation

¹ Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Abstract

We developed an in vivo serial passaging model for renal cancer with orthotopic renal subcapsular inoculation. We detail the procedures for renal subcapsular inoculation of cancer cells in mice, followed by in vivo and exvivo bioluminescence imaging, tumor-bearing kidney dissociation, and in vivo passaging. This protocol is capable of reproducing the coevolution between cancer cells and the primary tumor microenvironment. It enables us to unveil the systemic dynamics of metastasis and develop a therapeutic strategy for metastatic renal cancer. For complete details on the use and execution of this protocol, please refer to Nishida et al. (2020).

Keywords: Cancer; Cell Biology; Cell culture; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diagnostic Imaging
Female
Humans
Kidney / pathology
Kidney Neoplasms* / pathology
Male
Mice
Transplants* / pathology
Tumor Microenvironment