Three-phase partitioning, a recyclable method for the purification of R-phycoerythrin from a red algae Porphyra yezoensis

Prep Biochem Biotechnol. 2023;53(2):215-222. doi: 10.1080/10826068.2022.2065685. Epub 2022 May 2.


In this study, R-phycoerythrin (R-PE) was isolated and characterized from Porphyra yezoensis by three-phase partitioning (TPP) method. The effects of temperature, time, pH, salt saturation, and volume ratio on the purity and recovery rate of R-PE were studied. The optimum extraction conditions were determined as follows: salt saturation of 70%, temperature of 25 °C, time of 45 min, pH of 7.0, and volume ratio of 1:1. Under the optimal extraction conditions, the purity of R-PE was 3.90. The results of SDS-PAGE showed that R-PE has three bands at 23 kDa, 22 kDa, and 18 kDa, corresponding to its α, β, γ subunits. The structure and optical activity of R-PE did not change before and after purification based on ultraviolet, infrared, and fluorescence spectra. In addition, the purity and recovery rate of R-PE extracted by tert-butanol were evaluated. The results showed that the extraction performance of tert-butanol for R-PE remained unchanged in three recoveries. These show that TPP is an efficient, green, and recyclable extraction technology.

Keywords: Purification; R-phycoerythrin; recycling; three-phase partitioning.

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Phycoerythrin / chemistry
  • Porphyra*
  • Rhodophyta* / chemistry
  • tert-Butyl Alcohol


  • Phycoerythrin
  • tert-Butyl Alcohol