Construction and Characterization of HIV-1 env-Pseudoviruses of the Recombinant Form CRF63_02A and Subtype A6

N B Rudometova; N S Shcherbakova; D N Shcherbakov; O S Taranov; B N Zaitsev; L I Karpenko

doi:10.1007/s10517-022-05466-7

Construction and Characterization of HIV-1 env-Pseudoviruses of the Recombinant Form CRF63_02A and Subtype A6

Bull Exp Biol Med. 2022 Apr;172(6):729-733. doi: 10.1007/s10517-022-05466-7. Epub 2022 May 2.

Authors

N B Rudometova¹, N S Shcherbakova², D N Shcherbakov², O S Taranov², B N Zaitsev², L I Karpenko²

Affiliations

¹ State Research Center of Virology and Biotechnology Vector, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia. nadenkaand100@mail.ru.
² State Research Center of Virology and Biotechnology Vector, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia.

PMID: 35501651
DOI: 10.1007/s10517-022-05466-7

Abstract

HIV-1 env-pseudoviruses are a useful tool in the search for antiviral drugs (entry inhibitors) and evaluation of the efficacy of HIV-1 vaccines. Given the high genetic variability of HIV-1, it is necessary to regularly update the panels of pseudoviruses in accordance with the emergence of new strains. Based on genetic variants of HIV-1 circulating in the regions of the Siberian Federal District, 13 HIV-1 env-pseudoviruses of recombinant form CRF63_02A and subtype A6 were obtained. Most pseudoviruses have been shown to be sensitive to neutralization by bnAbs VRC01, PGT126, and 10E8, moderately sensitive to bnAbs PG9 and 4E10, and resistant to bnAbs 2G12, PG16, and 2F5. All obtained variants of pseudoviruses are CCR5-tropic.

Keywords: A6; CRF63_02A; broadly neutralizing antibodies (bnAbs); env-pseudoviruses; virus neutralization assay.

MeSH terms

Antibodies, Neutralizing
Broadly Neutralizing Antibodies
HIV Antibodies
HIV Infections*
HIV-1* / genetics
Humans
Neutralization Tests

Substances

Antibodies, Neutralizing
Broadly Neutralizing Antibodies
HIV Antibodies