In Vitro Generation of Heart Field Specific Cardiomyocytes

doi:10.1007/978-1-0716-1979-7_17

. 2022:2429:257-267.

doi: 10.1007/978-1-0716-1979-7_17.

In Vitro Generation of Heart Field Specific Cardiomyocytes

Arash Pezhouman^{1

2}, Ngoc B Nguyen^{1

2

3}, Allison Shevtsov¹, Rong Qiao¹, Reza Ardehali^{4

5

6

7}

Affiliations

¹ Division of Cardiology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
² Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA, USA.
³ Molecular, Cellular and Integrative Physiology Graduate Program, University of California, Los Angeles, Los Angeles, CA, USA.
⁴ Division of Cardiology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.
⁵ Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.
⁶ Molecular, Cellular and Integrative Physiology Graduate Program, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.
⁷ Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.

PMID: 35507167
DOI: 10.1007/978-1-0716-1979-7_17

In Vitro Generation of Heart Field Specific Cardiomyocytes

Arash Pezhouman et al. Methods Mol Biol. 2022.

. 2022:2429:257-267.

doi: 10.1007/978-1-0716-1979-7_17.

Authors

Arash Pezhouman^{1

2}, Ngoc B Nguyen^{1

2

3}, Allison Shevtsov¹, Rong Qiao¹, Reza Ardehali^{4

5

6

7}

Affiliations

¹ Division of Cardiology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
² Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA, USA.
³ Molecular, Cellular and Integrative Physiology Graduate Program, University of California, Los Angeles, Los Angeles, CA, USA.
⁴ Division of Cardiology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.
⁵ Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.
⁶ Molecular, Cellular and Integrative Physiology Graduate Program, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.
⁷ Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA. RArdehali@mednet.ucla.edu.

PMID: 35507167
DOI: 10.1007/978-1-0716-1979-7_17

Abstract

Myocardial infarction (MI) can lead to irreversible loss of cardiomyocytes (CMs), primarily localized to the left ventricle (LV) of the heart. The CMs of the LV are predominantly derived from first heart field (FHF) progenitors, whereas the majority of CMs within the right ventricle originate from the second heart field (SHF) during early cardiogenesis. Human embryonic stem cells (hESCs) serve as a valuable source of CMs for understanding early cardiac development and lineage commitment of CMs within these two heart fields that ultimately enable the development of more effective candidates for cell therapy. An ideal candidate may be FHF CMs that share the same ontogeny with the LV CMs that die after MI. We previously generated a double reporter hESC line that utilizes two important cardiac transcription factors, TBX5 and NKX2-5. TBX5 marks FHF progenitors and CMs, while NKX2-5 is expressed in nearly all myocytes of the developing heart. Here, we describe a step-by-step approach to efficiently generate FHF and SHF CMs using this double reporter hESC line. In addition, this approach can be applied to any non-genetically modified hESC lines to enrich FHF and SHF CMs. Obtaining enriched populations of these two CM subtypes provides a platform for downstream comparative analyses and in vitro studies to facilitate a deeper understanding of cardiovascular lineage commitment and the development of more effective candidates for cell therapy to treat diseases or defects that affect specific regions of the heart.

Keywords: Cardiac differentiation; First heart field cardiomyocyte; Human embryonic stem cell; Human heart development; Second heart field cardiomyocyte.

PubMed Disclaimer

Cited by

Tracing the history of a heart.
Spurlock B, Qian L. Spurlock B, et al. Elife. 2023 Jul 14;12:e89988. doi: 10.7554/eLife.89988. Elife. 2023. PMID: 37449466 Free PMC article.

References

1. Ilic D, Ogilvie C (2017) Concise review: human embryonic stem cells-what have we done? What are we doing? Where are we going? Stem Cells 35(1):17–25. https://doi.org/10.1002/stem.2450 - DOI - PubMed
1. Romagnuolo R, Masoudpour H, Porta-Sánchez A et al (2019) Human embryonic stem cell-derived cardiomyocytes regenerate the infarcted pig heart but induce ventricular tachyarrhythmias. Stem Cell Reports 12(5):967–981. https://doi.org/10.1016/j.stemcr.2019.04.005 - DOI - PubMed - PMC
1. Kim D, Kim SB, Ryu JL et al (2020) Human embryonic stem cell-derived Wilson's disease model for screening drug efficacy. Cells 9(4):872. https://doi.org/10.3390/cells9040872 - DOI - PMC
1. Crespo M, Vilar E, Tsai SY et al (2017) Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing. Nat Med 23(7):878–884. https://doi.org/10.1038/nm.4355 - DOI - PubMed - PMC
1. DeLaughter DM, Bick AG, Wakimoto H et al (2016) Single-cell resolution of temporal gene expression during heart development. Dev Cell 39(4):480–490. https://doi.org/10.1016/j.devcel.2016.10.001 - DOI - PubMed - PMC

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

Grants and funding

F31 HL144057/HL/NHLBI NIH HHS/United States

LinkOut - more resources

Full Text Sources
- Springer
Medical
- MedlinePlus Health Information

[1] Ilic D, Ogilvie C (2017) Concise review: human embryonic stem cells-what have we done? What are we doing? Where are we going? Stem Cells 35(1):17–25. https://doi.org/10.1002/stem.2450 - DOI - PubMed

[2] Ilic D, Ogilvie C (2017) Concise review: human embryonic stem cells-what have we done? What are we doing? Where are we going? Stem Cells 35(1):17–25. https://doi.org/10.1002/stem.2450 - DOI - PubMed

[3] Romagnuolo R, Masoudpour H, Porta-Sánchez A et al (2019) Human embryonic stem cell-derived cardiomyocytes regenerate the infarcted pig heart but induce ventricular tachyarrhythmias. Stem Cell Reports 12(5):967–981. https://doi.org/10.1016/j.stemcr.2019.04.005 - DOI - PubMed - PMC

[4] Romagnuolo R, Masoudpour H, Porta-Sánchez A et al (2019) Human embryonic stem cell-derived cardiomyocytes regenerate the infarcted pig heart but induce ventricular tachyarrhythmias. Stem Cell Reports 12(5):967–981. https://doi.org/10.1016/j.stemcr.2019.04.005 - DOI - PubMed - PMC

[5] Kim D, Kim SB, Ryu JL et al (2020) Human embryonic stem cell-derived Wilson's disease model for screening drug efficacy. Cells 9(4):872. https://doi.org/10.3390/cells9040872 - DOI - PMC

[6] Kim D, Kim SB, Ryu JL et al (2020) Human embryonic stem cell-derived Wilson's disease model for screening drug efficacy. Cells 9(4):872. https://doi.org/10.3390/cells9040872 - DOI - PMC

[7] Crespo M, Vilar E, Tsai SY et al (2017) Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing. Nat Med 23(7):878–884. https://doi.org/10.1038/nm.4355 - DOI - PubMed - PMC

[8] Crespo M, Vilar E, Tsai SY et al (2017) Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing. Nat Med 23(7):878–884. https://doi.org/10.1038/nm.4355 - DOI - PubMed - PMC

[9] DeLaughter DM, Bick AG, Wakimoto H et al (2016) Single-cell resolution of temporal gene expression during heart development. Dev Cell 39(4):480–490. https://doi.org/10.1016/j.devcel.2016.10.001 - DOI - PubMed - PMC

[10] DeLaughter DM, Bick AG, Wakimoto H et al (2016) Single-cell resolution of temporal gene expression during heart development. Dev Cell 39(4):480–490. https://doi.org/10.1016/j.devcel.2016.10.001 - DOI - PubMed - PMC

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

In Vitro Generation of Heart Field Specific Cardiomyocytes

Affiliations

In Vitro Generation of Heart Field Specific Cardiomyocytes

Authors

Affiliations

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Medical