In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay

Nick A Kuburich; Petra den Hollander; Abhijeet P Deshmukh; Suhas Vasaikar; Robiya Joseph; Max S Wicha; Sendurai A Mani

doi:10.1007/978-1-0716-1979-7_35

In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay

Methods Mol Biol. 2022:2429:509-513. doi: 10.1007/978-1-0716-1979-7_35.

Authors

Nick A Kuburich¹, Petra den Hollander¹, Abhijeet P Deshmukh¹, Suhas Vasaikar¹, Robiya Joseph¹, Max S Wicha², Sendurai A Mani³

Affiliations

¹ Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
² Forbes Institute for Cancer Discovery, University of Michigan Medical School, Ann Arbor, MI, USA.
³ Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. smani@mdanderson.org.

PMID: 35507185
DOI: 10.1007/978-1-0716-1979-7_35

Abstract

Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. In this chapter, we provide a detailed method for the quantification of CSCs in vitro through mammosphere formation.

Keywords: Cancer stem cells (CSC); Mammosphere; Stemness; Suspension; Tumorsphere.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Breast Neoplasms* / pathology
Cell Line, Tumor
Female
Humans
Neoplastic Stem Cells* / pathology

Abstract

Publication types

MeSH terms

Grants and funding