Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 95 (1), 85-7

Failure to Detect Hydrogen-Sulphide Production in Lactose/Sucrose-Fermenting Enterobacteriaceae, Using Triple Sugar Iron Agar

Failure to Detect Hydrogen-Sulphide Production in Lactose/Sucrose-Fermenting Enterobacteriaceae, Using Triple Sugar Iron Agar

H J Kolmos et al. Acta Pathol Microbiol Immunol Scand B.

Abstract

Triple Sugar Iron agar failed to detect hydrogen sulphide in 44 out of 69 hydrogen-sulphide producing strains of Enterobacteriaceae, which at the same time fermented lactose and/or sucrose. The species involved were Salmonella typhi, Salmonella enteritidis, Citrobacter freundii, Escherichia coli, and Proteus vulgaris. By contrast, no false-negative reactions were observed in 74 strains, which fermented neither lactose nor sucrose. Failure to detect hydrogen sulphide was probably due to acidification of the medium following the fermentation of carbohydrates. A medium without carbohydrates is preferable in diagnostic situations where hydrogen-sulphide detection is of great importance.

Similar articles

See all similar articles

Cited by 2 PubMed Central articles

LinkOut - more resources

Feedback