First report demonstrating the presence of Toxocara spp. eggs on vegetables grown in community gardens in Europe

Sara R Healy; Eric R Morgan; Joaquin M Prada; Martha Betson

doi:10.1016/j.fawpar.2022.e00158

First report demonstrating the presence of Toxocara spp. eggs on vegetables grown in community gardens in Europe

Food Waterborne Parasitol. 2022 Apr 20:27:e00158. doi: 10.1016/j.fawpar.2022.e00158. eCollection 2022 Jun.

Authors

Sara R Healy¹, Eric R Morgan², Joaquin M Prada¹, Martha Betson¹

Affiliations

¹ University of Surrey, School of Veterinary Medicine, Daphne Jackson Road, Guildford, Surrey GU2 7AL, UK.
² Queen's University, Institute for Global Food Security, Biological Sciences Building, 19 Chlorine Gardens, BT9 5DL Belfast, UK.

Abstract

Toxocara canis and T. cati are zoonotic roundworm parasites of dogs, cats and foxes. These definitive hosts pass eggs in their faeces, which contaminate the environment and can subsequently be ingested via soil or contaminated vegetables. In humans, infection with Toxocara can have serious health implications. This proof-of-concept study aimed to investigate the presence of Toxocara spp. eggs on 'ready-to-eat' vegetables (lettuce, spinach, spring onion and celery) sampled from community gardens in southern England. The contamination of vegetables with Toxocara eggs has never been investigated in the UK before, and more widely, this is the first time vegetables grown in community gardens in Europe have been assessed for Toxocara egg contamination. Sixteen community gardens participated in the study, providing 82 vegetable samples fit for analysis. Study participants also completed an anonymous questionnaire on observed visits to the sites by definitive hosts of Toxocara. Comparison of egg recovery methods was performed using lettuce samples spiked with a series of Toxocara spp. egg concentrations, with sedimentation and centrifugal concentration retrieving the highest number of eggs. A sample (100 g) of each vegetable type obtained from participating community gardens was tested for the presence of Toxocara eggs using the optimised method. Two lettuce samples tested positive for Toxocara spp. eggs, giving a prevalence of 2.4% (95% CI =1.3-3.5%) for vegetable samples overall, and 6.5% (95% CI = 4.7-8.3%; n = 31) specifically for lettuce. Questionnaire data revealed that foxes, cats and dogs frequently visited the community gardens in the study, with 88% (68/77) of respondents reporting seeing a definitive host species or the faeces of a definitive host at their site. This proof-of-concept study showed for the first time the presence of Toxocara spp. eggs on vegetables grown in the UK, as well as within the soil where these vegetables originated, and highlights biosecurity and zoonotic risks in community gardens. This study establishes a method for assessment of Toxocara spp. eggs on vegetable produce and paves the way for larger-scale investigations of Toxocara spp. egg contamination on field-grown vegetables.

Keywords: Allotments; Community gardens; Food safety; Public health; Toxocara; Vegetables; Zoonosis.