Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA

Biochem Biophys Res Commun. 1987 Mar 13;143(2):740-8. doi: 10.1016/0006-291x(87)91416-1.


A lambda gt10 library containing DNAs complementary to messenger RNAs from human breast cancer T47-D cells was constructed and screened with a cDNA probe encoding the rabbit progesterone receptor. Four overlapping clones have been sequenced. The open reading frame corresponds to a protein of 933 amino acids with a molecular weight of 98,868 Da. The cysteine rich basic region supposed to be involved in DNA binding is completely homologous in the human and rabbit receptors, whereas the C-terminal end, where hormone binding is thought to take place, differs by a single amino acid change. The human progesterone receptor is characterized, as is the rabbit receptor, by the very high proline content of its N-terminal region. When mRNAs from either human breast cancer cell lines T47-D and MCF-7 or from normal human uterus tissue were blotted and probed with the cloned cDNA, four main bands were observed (5100, 4300, 3700, and 2900 nucleotides).

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Breast Neoplasms
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics
  • Female
  • Gene Expression Regulation
  • Humans
  • Proto-Oncogene Proteins
  • RNA, Messenger / genetics
  • Receptors, Glucocorticoid
  • Receptors, Progesterone* / genetics
  • Uterus / physiology


  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Receptors, Glucocorticoid
  • Receptors, Progesterone
  • DNA

Associated data

  • GENBANK/M15716