Pediococcus acidilactici FZU106 alleviates high-fat diet-induced lipid metabolism disorder in association with the modulation of intestinal microbiota in hyperlipidemic rats

Qing Zhang; Wei-Ling Guo; Gui-Mei Chen; Min Qian; Jin-Zhi Han; Xu-Cong Lv; Li-Jiao Chen; Ping-Fan Rao; Lian-Zhong Ai; Li Ni

doi:10.1016/j.crfs.2022.04.009

Pediococcus acidilactici FZU106 alleviates high-fat diet-induced lipid metabolism disorder in association with the modulation of intestinal microbiota in hyperlipidemic rats

Curr Res Food Sci. 2022 Apr 27:5:775-788. doi: 10.1016/j.crfs.2022.04.009. eCollection 2022.

Authors

Qing Zhang^{1

2}, Wei-Ling Guo^{1

3}, Gui-Mei Chen¹, Min Qian⁴, Jin-Zhi Han^{1

5}, Xu-Cong Lv^{1

5}, Li-Jiao Chen², Ping-Fan Rao^{1

5}, Lian-Zhong Ai⁶, Li Ni^{1

5}

Affiliations

¹ Institute of Food Science and Technology, College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350108, PR China.
² College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China.
³ International Joint Research Center for Probiotics & Gut Health, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, PR China.
⁴ College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, PR China.
⁵ Food Nutrition and Health Research Center, School of Advanced Manufacturing, Fuzhou University, Jinjiang, Fujian, 362200, PR China.
⁶ School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, PR China.

Abstract

Probiotics have been proved to have beneficial effects in improving hyperlipidemia. The purpose of the current research was to investigate the ameliorative effects of Pediococcus acidilactici FZU106, isolated from the traditional brewing of Hongqu rice wine, on lipid metabolism and intestinal microbiota in high-fat diet (HFD)-induced hyperlipidemic rats. Results showed that P. acidilactici FZU106 intervention obviously inhibited the abnormal increase of body weight, ameliorated serum and liver biochemical parameters related to lipid metabolism and oxidative stress. Histopathological evaluation also showed that P. acidilactici FZU106 could significantly reduce the excessive lipid accumulation in liver caused by HFD-feeding. Furthermore, P. acidilactici FZU106 intervention significantly increased the short-chain fatty acids (SCFAs) levels in HFD-fed rats, which was closely related to the changes of intestinal microbial composition and metabolism. Intestinal microbiota profiling by high-throughput sequencing demonstrated that P. acidilactici FZU106 intervention evidently increased the proportion of Butyricicoccus, Pediococcus, Rothia, Globicatella and [Eubacterium]_coprostanoligenes_group, and decreased the proportion of Corynebacterium_1, Psychrobacter, Oscillospira, Facklamia, Pseudogracilibacillus, Clostridium_innocuum_group, Enteractinococcus and Erysipelothrix in HFD-fed rats. Additionally, P. acidilactici FZU106 significantly regulated the mRNA levels of liver genes (including CD36, CYP7A1, SREBP-1c, BSEP, LDLr and HMGCR) involved in lipid metabolism and bile acid homeostasis. Therefore, these findings support the possibility that P. acidilactici FZU106 has the potential to reduce the disturbance of lipid metabolism by regulating intestinal microflora and liver gene expression profiles.

Keywords: Hyperlipidemia; Intestinal microbiota; Lipid metabolism; Pediococcus acidilactici FZU106; mRNA expression.