Engineering multiscale structural orders for high-fidelity embryoids and organoids

Yue Shao; Jianping Fu

doi:10.1016/j.stem.2022.04.003

Engineering multiscale structural orders for high-fidelity embryoids and organoids

Cell Stem Cell. 2022 May 5;29(5):722-743. doi: 10.1016/j.stem.2022.04.003.

Authors

Yue Shao¹, Jianping Fu²

Affiliations

¹ Institute of Biomechanics and Medical Engineering, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, China; State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: yshao@tsinghua.edu.cn.
² Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Cell & Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

PMID: 35523138
PMCID: PMC9097334 (available on 2023-05-05)
DOI: 10.1016/j.stem.2022.04.003

Abstract

Embryoids and organoids hold great promise for human biology and medicine. Herein, we discuss conceptual and technological frameworks useful for developing high-fidelity embryoids and organoids that display tissue- and organ-level phenotypes and functions, which are critically needed for decoding developmental programs and improving translational applications. Through dissecting the layers of inputs controlling mammalian embryogenesis, we review recent progress in reconstructing multiscale structural orders in embryoids and organoids. Bioengineering tools useful for multiscale, multimodal structural engineering of tissue- and organ-level cellular organization and microenvironment are also discussed to present integrative, bioengineering-directed approaches to achieve next-generation, high-fidelity embryoids and organoids.

Keywords: developmental biology; embryoid; mammalian embryogenesis; mechanobiology; organoid; stem cell engineering.

Publication types

Review
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bioengineering*
Embryo, Mammalian
Mammals
Organoids*
Tissue Engineering

Abstract

Publication types

MeSH terms

Grant support