Discovery of brevijanazines from Aspergillus brevijanus reveals the molecular basis for p-nitrobenzoic acid in fungi

Chem Commun (Camb). 2022 May 26;58(43):6296-6299. doi: 10.1039/d2cc01679f.


The brevijanazines are novel p-nitrobenzoylated piperazines isolated from Aspergillus brevijanus. Their structures were elucidated by spectroscopic analysis, X-ray crystallography and total synthesis. Heterologous biosynthesis, precursor feeding and in vitro microsomal assays unveiled the biosynthetic pathway to the brevijanazines, featuring a cytochrome P450 oxygenase that converts p-aminobenzoic acid to p-nitrobenzoic acid.

MeSH terms

  • Aspergillus*
  • Biosynthetic Pathways
  • Fungi* / chemistry
  • Molecular Structure
  • Nitrobenzoates


  • Nitrobenzoates
  • 4-nitrobenzoic acid

Supplementary concepts

  • Aspergillus brevijanus