Cytopathogenic effect of Salmonella typhi GIFU 10007 on M cells of murine ileal Peyer's patches in ligated ileal loops: an ultrastructural study

Microbiol Immunol. 1986;30(12):1225-37. doi: 10.1111/j.1348-0421.1986.tb03055.x.


An electron microscopic study revealed that, within 30 min after inoculation into the ligated ileal loop of anesthetized mice, cells of Salmonella typhi GIFU 10007 adhered to the M cell surface of Peyer's patch lymphoid follicle epithelium, and induced almost complete destruction of M cells. The M cell cytoplasms were pinched off and extruded from the epithelial lining into the luminal space together with the lymphoid cells primarily enfolded into the corresponding M cells. When two or more M cells were destroyed, a large defect in the epithelial lining was apparent, and a number of bacteria appeared near the basal lamina of the epithelial lining. These findings suggest, as far as anesthetized murine ileal loops and strain 10007 are concerned, that ileal M cells are the target cell at an early stage of S. typhi infection and the infection may further progress to deeper tissues and to the general circulation.

MeSH terms

  • Animals
  • Bacterial Adhesion
  • Fluorescent Antibody Technique
  • Ileum
  • Mice
  • Microscopy, Electron
  • Microscopy, Electron, Scanning
  • Microvilli / microbiology
  • Microvilli / ultrastructure
  • Peyer's Patches / cytology
  • Peyer's Patches / microbiology*
  • Peyer's Patches / ultrastructure
  • Salmonella typhi / pathogenicity*
  • Salmonella typhi / ultrastructure