ACI/EG eutectic mixture mediated synthesis, characterization and in vitro osteoblast differentiation assessment of spiropyrrolo[1,2- b]isoquinoline analogues

Govindasami Periyasami; Natarajan Arumugam; Mostafizur Rahaman; Raju Suresh Kumar; Muthurangan Manikandan; Musaad A Alfayez; Dhanaraj Premnath; Ali Aldalbahi

doi:10.1039/c8ra00646f

ACI/EG eutectic mixture mediated synthesis, characterization and in vitro osteoblast differentiation assessment of spiropyrrolo[1,2- b]isoquinoline analogues

RSC Adv. 2018 May 2;8(29):16303-16313. doi: 10.1039/c8ra00646f. eCollection 2018 Apr 27.

Authors

Govindasami Periyasami^{1

2}, Natarajan Arumugam¹, Mostafizur Rahaman¹, Raju Suresh Kumar¹, Muthurangan Manikandan³, Musaad A Alfayez³, Dhanaraj Premnath⁴, Ali Aldalbahi¹

Affiliations

¹ Department of Chemistry, College of Science, King Saud University P.O. Box 2455 Riyadh 11451 Saudi Arabia aaldalbahi@ksu.edu.sa pkandhan@ksu.edu.sa.
² Department of Organic Chemistry, University of Madras, Guindy Campus Chennai 600 025 India.
³ Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University Riyadh Saudi Arabia.
⁴ Department of Oral Biology Dentistry Building-D325 Winnipeg Manitoba Canada.

Abstract

An eco-friendly acetylcholine iodide-ethylene glycol (ACI/EG) deep eutectic mixture mediated green protocol has been developed for the synthesis of hitherto unexplored multi-functionalized linear tricyclic spiropyrrolo[1,2-b]isoquinoline analogues. The effects of the synthesized compounds on the osteoblast differentiation of hBMSC-TERT cell lines were investigated and promising results were observed with significant IC₅₀ values. In addition, molecular modeling simulations were also performed with the 3D structure of BMP-2 to reveal binding interactions and orientations of highly potent spiropyrrolo[1,2-b]isoquinoline analogues.