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. 2022 Dec;16(1):65-71.
doi: 10.1080/19336918.2022.2072554.

Triple-negative and triple-positive breast cancer cells reciprocally control their growth and migration via the S100A4 pathway

Elena A Dukhanina  1 , Tatiana N Portseva  1 , Alexander S Dukhanin  2 , Sofia G Georgieva  1
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Triple-negative and triple-positive breast cancer cells reciprocally control their growth and migration via the S100A4 pathway

Elena A Dukhanina et al. Cell Adh Migr. 2022 Dec.

Abstract

The study's aim was to investigate the S100A4-mediated mechanisms of the regulation of tumor cell proliferation and migration in the human triple-positive breast carcinoma cell line MCF-7 (TPBC) and triple-negative breast carcinoma cell line MDA-MB-231 (TNBC). The proliferative activity of TNBC more than doubled during the incubation in the conditioned medium of TPBC. Extracellular S100A4 dose-dependently decreased the proliferative response of TPBC. TPBC negatively impacted the growth of TNBCs during their co-culturing. TPBC significantly decreased the migration activity of the TNBC cells while the S100A4 intracellular level in the TNBC was also decreasing. The decrease in the S100A4 intracellular level occurred due to the protein's monomeric form while the contribution of the dimeric form into the overall S100A4 concentration in TNBC cells increased 1.5-2-fold. The S100A4 pathway in the intercellular communication between TNBC and TPBCs also included the dexamethasone-sensitive mechanisms of S100A4 intra- and extracellular pools regulation.

Keywords: Proliferative activity; S100A4; cell target; dexamethasone; migration.

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Conflict of interest statement

No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
The proliferative activity of TPBC. The cells were incubated with: 1 – TPBC-medium; 2 – TNBC-medium; and 3 – S-medium (stimulated conditioned medium), *p < 0.05.B. The proliferative activity of TNBC without (black boxes) and under the presence (gray boxes) of dexamethasone (1 μmol/l): 1 – TNBC-medium; 2 – TPBC-medium; and 3 – S-medium, *p < 0.05.C. (Left panel, 4 boxes) The influence of S100A4 on the TNBC proliferative. activity: in TPBC-medium and under the 24-hour treatment by S100A4 in the concentration of 10, 20, and 40 ng per thousand cells;(Right panel, 2 boxes) The influence of S100A4 on the TNBC proliferative activity in TNBC-medium and under the 24-hour treatment by S100A4 in the concentration of 10 ng.D. The influence of S100A4 on TPBC growth. The proliferative activity of.TPBC was measured in TPBC-medium and under the 24-hour treatment by S100A4 in the concentration of 10, 20, and 40 ng per thousand cells.
Figure 2.
Figure 2.
The measurement of intra- and extracellular S100A4 concentrations in TNBC and the migration activity of TNBC.
See this image and copyright information in PMC

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The work was carried out within the framework of the State task of the Ministry of Science and Higher Education of the Russian Federation (state registration of the topic - #121052600288-5).