Physicochemical transformation and enzymatic hydrolysis promotion of reed straw after pretreatment with a new deep eutectic solvent

Zhaohui Zhang; Jun Xu; Junxian Xie; Shiyun Zhu; Bin Wang; Jun Li; Kefu Chen

doi:10.1016/j.carbpol.2022.119472

Physicochemical transformation and enzymatic hydrolysis promotion of reed straw after pretreatment with a new deep eutectic solvent

Carbohydr Polym. 2022 Aug 15:290:119472. doi: 10.1016/j.carbpol.2022.119472. Epub 2022 Apr 10.

Authors

Zhaohui Zhang¹, Jun Xu², Junxian Xie¹, Shiyun Zhu³, Bin Wang⁴, Jun Li¹, Kefu Chen¹

Affiliations

¹ Plant Fiber Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangzhou 510006, China.
² Plant Fiber Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangzhou 510006, China; Qingyuan Huayuan Institute of Science and Technology Collaborative Innovation Co., Ltd., Qingyuan 511500, China. Electronic address: xujun@scut.edu.cn.
³ Plant Fiber Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangzhou 510006, China. Electronic address: zhushiyun1992@scut.edu.cn.
⁴ Plant Fiber Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, Guangzhou 510006, China; Qingyuan Huayuan Institute of Science and Technology Collaborative Innovation Co., Ltd., Qingyuan 511500, China.

PMID: 35550767
DOI: 10.1016/j.carbpol.2022.119472

Abstract

This work proposed a promising biorefinery method for the deconstruction of reed straw by using a novel deep eutectic solvent (DES) consisted of benzyltriethylammonium chloride/formic acid (BTEAC/FA). BTEAC/FA showed significant delignification and xylan removal while preserving most of the cellulose. Under the optimum conditions (molar ratio: 1:6), the glucose yield of cellulose-rich substrates by enzymatic hydrolysis reached 76.64%, which was about 5.24-fold higher than that of raw reed straw. The removal of lignin and hemicellulose and the changes in the cellulose crystal structure were presumed to the reason for enhancing enzymatic hydrolysis efficiency. The highest lignin yield reached 78.34%. Moreover, the lignin exhibited high purity (84.25%-91.45%), medium molecular weight (3812-9918 g/mol), and low polydispersity (1.47-2.75) because of the cleavage of β-O-4 and β-β linkages. Overall, this work provided a theoretical basis for the in-depth utilization of reed straw.

Keywords: Benzyltriethylammonium chloride/formic acid; Deep eutectic solvent; Enzymatic hydrolysis; Fractionation; Reed straw.

MeSH terms

Biomass
Cellulose
Deep Eutectic Solvents*
Hydrolysis
Lignin* / chemistry
Solvents / chemistry

Substances

Deep Eutectic Solvents
Solvents
Cellulose
Lignin