A method for preparing leucocyte depleted erythrocytes from rat blood

J Immunol Methods. 1978;22(3-4):389-91. doi: 10.1016/0022-1759(78)90047-9.


By centrifuging heparinised rat blood 2 or 3 times through double discontinuous Ficoll--Hypaque density density gradients it is possible to achieve over 99.8% depletion of leucocytes and to retrieve nearly all the erythrocytes and leucocytes in the original specimen.

MeSH terms

  • Animals
  • Cell Separation
  • Centrifugation, Density Gradient
  • Erythrocytes / immunology*
  • Immunologic Techniques
  • Leukocytes / immunology*
  • Rats