Proteomic analysis of Plasmodium berghei in the ring phase during in vivo antiparasitic treatment with kramecyne

Exp Parasitol. 2022 Jul;238:108262. doi: 10.1016/j.exppara.2022.108262. Epub 2022 May 10.

Abstract

Malaria is a parasitic disease of global importance due to its high annual death toll. The treatment for this infection is difficult for the increase in the populations of parasites resistant to the existing medicines, the development of new antimalarials is urgent needed. Several products developed for the control of malaria from herbalist have had a profound impact, for example, quinine obtained from the bark of the cinchona tree and recently those derived from artemisinin, whose discovery was the reason for the awarding of the 2015 Nobel Prize. The aim of the present study was to evaluate a compound named kramecyne extracted of "chayotillo" (Krameria cystisoides) plant used by the antiparasitic effect against some blood and intestinal protozoa (Giardia duodenalis y Trypanosoma cruzi). In addition is using for the treatment of inflammatory diseases. Measuring parasitaemia at different times, it was observed that in mice treated with kramecyne, it reached only 14% of parasitaemia at 7 days with a dose of 15 mg/kg, using chloroquine as a control drug, because it has not been demonstrated that parasites that infect rodents have developed resistance against this drug. Our results showed that kramecyne decreases the expression of parasite proteins that participate in biological processes, such as invasion, cytoadherence, pathogenicity and energy metabolism. With these results, it is proposed that this compound has repercussions on the metabolism of the parasite and could be useful for use as an antimalarial.

Keywords: Antimalarial; Mass spectrometry; Metabolic networks; Plasmodium berghei; kramecyne; malaria.

MeSH terms

  • Animals
  • Antimalarials* / pharmacology
  • Antimalarials* / therapeutic use
  • Antiparasitic Agents / pharmacology
  • Ethers, Cyclic
  • Malaria* / drug therapy
  • Malaria* / parasitology
  • Mice
  • Peroxides
  • Plant Extracts / pharmacology
  • Plasmodium berghei
  • Plasmodium falciparum
  • Proteomics

Substances

  • Antimalarials
  • Antiparasitic Agents
  • Ethers, Cyclic
  • Peroxides
  • Plant Extracts
  • kramecyne