Trans-3'-hydroxycotinine as a main metabolite in urine of smokers

Int Arch Occup Environ Health. 1987;59(2):199-201. doi: 10.1007/BF00378497.


Nine male smokers smoked three types of cigarettes that differed in mainstream nicotine. The experiment was carried out in three runs, each consisting of 7 d when smoking was allowed and 5 d when it was not allowed. Urine fractions were collected throughout the whole period of the experiments. As assumed, nicotine and four of its known metabolites, i.e., cotinine, nicotine-N'-oxide, nornicotine, and N-methyl-nicotinum ions were found in the urine. In addition, trans-3'-hydroxycotinine was determined. The amount of this metabolite was well in excess of those of nicotine and cotinine in the urine. The serum concentration trans-3'-hydroxycotinine was found to be second to serum cotinine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cotinine / analogs & derivatives
  • Cotinine / urine*
  • Humans
  • Male
  • Nicotine / metabolism
  • Nicotine / urine
  • Pyrrolidinones / urine*
  • Smoking*


  • Pyrrolidinones
  • hydroxycotinine
  • Nicotine
  • Cotinine